NOX2/gp91phox Monoclonal Antibody

SKU: orb76741

Description

NOX2/gp91phox Monoclonal Antibody

Research Area

Metabolism Research

Images & Validation

• Tested Applications: ICC, IHC-P, WB
• Dilution Range: Optimal antibody concentration should be determined by titration, however as a guideline try;WB: Use at an assay dependent dilution. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).ICC/IF: Use a concentration of 1 µg/ml.IHC-P: Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol
• Reactivity: Human
• Application Notes:
  SP2/0

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 54.1
• Immunogen: Partially Purified human neutrophil flavocytochrome b (heparin Ultrogellectin affinity purification in Triton X-100)
• Target: NOX2/gp91phox
• Purification: Protein G
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Purified antibody containing PBS 0.1% sodium azide
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CGD91-phox antibody;CYBB antibody;Cytochrome b(558) beta chain antibody;Cytochrome b-245 heavychain antibody;gp91-1 antibody;gp91-phox antibody;Heme-binding membrane glycoprotein gp91phox antibody;NADPHoxidase 2 antibody;Neutrophil cytochrome b 91 kDa polypeptide antibody;NOX2 antibody;p22 phagocyte B-cytochromeantibody;Superoxide-generating NADPH oxidase heavy chain subunit antibody

Western blot analysis of A431 cell line lysates using gp91phox antibody

Quick Database Links

Gene Symbol

NOX2/gp91phox

UniProt

P04839

NCBI Gene ID

1536