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Item 1 of 3
NOX1 Antibody (Center)
Catalog Number: orb1934249
| Catalog Number | orb1934249 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB36700 |
| Tested applications | IHC-P, WB |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | WB: 1:1000, IHC-P-Leica: 1:500, IHC-P-Leica: 1:500 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 64871 Da |
| Target | This NOX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 243-271 amino acids from the Central region of human NOX1. |
| UniProt ID | Q9Y5S8 |
| NCBI | NP_001258744.1, NP_008983.2, NP_039249.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | NADPH oxidase 1, NOX-1, 1---, Mitogenic oxidase 1, Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Immunohistochemical analysis of paraffin-embedded human colon tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Immunohistochemical analysis of paraffin-embedded human kidney tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
All lanes: Anti-NOX1 Antibody (Center) at 1:1000 dilution. Lane 1: WiDr whole cell lysate. Lane 2: SW480 whole cell lysate. Lane 3: HT-29 whole cell lysate. Lane 4: PC-3 whole cell lysate. Lane 5: Hela whole cell lysate. Lane 6: human uterus tissue lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 65 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
