NOX1 Antibody (Center)

SKU: orb1934249

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Metabolism Research

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB - 1:1000, IHC-P-Leica - 1:500
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD58922
• Immunogen: This NOX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 243-271 amino acids from the Central region of human NOX1. Antigen Region: 243-271 aa.
• Target: NOX1 (HGNC:7889)
• Molecular Weight: 64871 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NADPH oxidase 1, NOX-1, 1---, Mitogenic oxidase 1, MOX-1, NADH/NADPH mitogenic oxidase subunit P65-MOX, NOH-1, NOX1, MOX1, NOH1

Immunohistochemical analysis of paraffin-embedded human colon tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Immunohistochemical analysis of paraffin-embedded human kidney tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

All lanes: Anti-NOX1 Antibody (Center) at 1:1000 dilution. Lane 1: WiDr whole cell lysate. Lane 2: SW480 whole cell lysate. Lane 3: HT-29 whole cell lysate. Lane 4: PC-3 whole cell lysate. Lane 5: Hela whole cell lysate. Lane 6: human uterus tissue lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 65 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

NOX1 (HGNC:7889)

UniProt

Q9Y5S8

RefSeq (Protein):NP_008983.2, NP_039249.1, NP_001258744.1