NOTCH3 Antibody (C-term Q2306)

• Catalog Number: orb1939269
• Category: Antibodies
• Description: Mouse Monoclonal Antibody (Mab)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 487CT6.9.2
• Tested applications: WB
• Reactivity: Human
• Isotype: IgG1
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:2000, WB: 1:500~1000
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 243631 Da
• Target: This NOTCH3 antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 2291-2321 amino acids from the C-terminal region of human NOTCH3.
• UniProt ID: Q9UM47
• NCBI: NP_000426.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Neurogenic locus notch homolog protein 3, Notch 3,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-NOTCH3 Antibody at 1:2000 dilution + Hela whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 250 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

NOTCH3 Antibody (Q2306) western blot analysis in Hela cell line lysates (35 μg/lane). This demonstrates the NOTCH3 antibody detected the NOTCH3 protein (arrow).