Notch1 Recombinant Rabbit Monoclonal Antibody

SKU: orb2283907

Description

Notch1 Recombinant Rabbit Monoclonal Antibody

Research Area

Cardiovascular Research, Developmental Biology, Molecular Biology, Neuroscience, Notch Signaling Pathway, Surface Molecules

Images & Validation

• Tested Applications: FC, ICC, IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:50-200, IHC-F=1:50-200, IF=1:50-200, ICC/IF=1:50-200, Flow-Cyt=1:50-100
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: IgG
• Clone No.: 16B1
• Immunogen: A synthesized peptide derived from human Notch 1 (2500-2555/2555aa)
• Target: NOTCH1
• Molecular Weight: 271 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Neurogenic locus notch homolog protein 1; Notch 1 extracellular truncation; Notch 1 intracellular domain; hN1; Lin-12; LIN12; MIS6; Motch A; mT14; Neurogenic locus notch homolog protein 1; Neurogenic locus notch protein homolog; NICD; Notch1 intracellular domain; NOTC1_HUMAN; Notch 1; NOTCH; Notch gene homolog 1(Drosophila); Notch homolog 1 translocation associated(Drosophila); Notch homolog 1, translocation-associated(Drosophila); NOTCH, Drosophila, homolog of, 1; p300; TAN 1; TAN1; Translocation Associated Notch Homolog; Translocation Associated Notch Homolog; Translocation associated notch protein TAN 1; Translocation-associated notch protein TAN-1.

Paraformaldehyde-fixed, paraffin embedded (human pancreas), Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (Notch1) Monoclonal Antibody, Unconjugated (orb2283907) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse pancreas), Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (Notch1) Monoclonal Antibody, Unconjugated (orb2283907) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Quick Database Links

Gene Symbol

NOTCH1

UniProt

P46531