NOTCH2 Antibody

SKU: orb750486

Description

NOTCH2 antibody

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:20,000 - 1:100,000, IHC: 1:500, WB: 1:400 - 1:2,000
• Reactivity: Human
• Application Notes:
  Anti-Notch2 has been tested in ELISA, WB, and IHC. Anti-NOTCH-2 has a strong response was detected by ELISA against the immunizing peptide. This product was assayed against the peptide immunogen in a standard capture ELISA using Peroxidase conjugated anti-Rabbit IgG [H&L] (Goat) and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:30,000 to 1:90,000 is suggested in ELISA this product.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Antiserum
• Immunogen: This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to amino acid residues of human Notch 2 located near the N-terminal sequence of the cleaved N intracellular domain (NICD).
• Target: NOTCH2
• Purity: This antiserum is directed against human NOTCH2. The immunogen epitope is only exposed after gamma secretase cleavage and is not accessible in the uncleaved form. Based on sequences, we expect this antibody to react as well with rat and mouse NOTCH 2. Only one amino acid difference (conservative) occurs in mouse and rat.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 75 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Notch2 antibody, AGS2 antibody, hN2 antibody, Notch homolog 2 antibody, neurogenic locus notch homolog protein 2

Biorbyt's anti-NOTCH 2 antibody was diluted 1:500 to detect NOTCH 2 in human liver tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.

Western Blot of Rabbit Anti-Notch 2 N-Term Antibody. Lane 1: Opal Prestained Molecular Weight Marker. Lane 2: HeLa Whole Cell Lysate (p/n orb348668). Lane 3: MOLT-4 Whole Cell Lysate (p/n orb348698). Lane 4: K-562 Whole Cell Lysate (p/n orb348692). Lane 5: Human Kidney Lysate. Load: 20 µg lysate/lane. Primary Antibody: Anti-NOTCH 2 at 1:1000 for 1 hr at RT. Secondary Antibody: Goat Anti-Rabbit IgG HRP (p/n orb347654) at 1:70000 for 30 mins at RT. Blocking: 5% BLOTTO. Expected MW: ~86kDa.

Western Blot of Rabbit anti-NOTCH2 (Cleaved N terminal) antibody. Lane 1 Marker: Opal Pre-stained ladder. Lane 2: U-251 Lysate (p/n orb692720). Lane 3: PC-3 Lysate (p/n orb692718). Lane 4: MCF-7 Lysate (p/n orb348664). Lane 5: Hep-G2 Lysate (p/n orb348735). Lane 6: A431 Lysate (p/n orb348665). Lane 7: A549 Lysate (p/n orb348675). Lane 8: J774A.1 Lysate. Lane 9: C2C12 Lysate (p/n orb348725). Lane 10: Mouse Embryonic Fibroblast Lysate (p/n orb348722). Lane 11: NIH/3T3 Lysate (p/n orb348714). Lane 12: PC-12 Lysate (p/n orb348733). Lane 13: Rat Brain Lysate (p/n orb348727). Lane 14: Mouse Brain Lysate (p/n orb348715). Lane 15 Marker: Opal Pre-stained Ladder. Load: 10 µg of lysate/lane. Primary antibody: NOTCH2 antibody at 1 µg/ml overnight at 4°C. Secondary antibody: Peroxidase goat anti-rabbit secondary antibody (p/n orb347654) at 1:70000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS for 30 min at RT. Predicted size: ~86kDa in human cell lines (2-7) for NOTCH2.

Quick Database Links

Gene Symbol

NOTCH2

UniProt

Q04721

RefSeq:24041035