NOS1 Antibody

SKU: orb699411

Description

Mouse monoclonal antibody to NOS1

Research Area

Cancer Biology, Cell Biology, Neuroscience

Images & Validation

• Tested Applications: ELISA, ICC, IF, IHC, WB
• Dilution Range: WB (1:1000)
• Reactivity: Human, Mouse, Rat
• Application Notes:
  A 1:1000 dilution of SMC-609 was sufficient for detection of NOS1/Nnos in 7.5 ug of Mouse Brain Cerebellum by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone No.: J23
• Immunogen: Partial Human Recombinant NOS1 Protein
• Target: NOS1
• Molecular Weight: 160 kDa
• Purification: Protein A Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.09% sodium azide Storage buffer changes when conjugated
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

BNOS, Constitutive NOS, IHPS 1, IHPS1, N-NOS, NC-NOS, neuronal Nitric Oxide Synthase, Neuronal NOS, Nitric oxide synthase neuronal included, Nitric oxide synthase 1 (neuronal), Nitric oxide synthase 1, NNOS, NO, NOS 1, NOS, NOS type I, NOS-I, NOS1, NOS1_HUMAN

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-NOS1/ nNos Monoclonal Antibody, Clone J23. Tissue: Differentiated SH-SY5Y. Species: Human. Primary Antibody: Mouse Anti-NOS1/ nNos Monoclonal Antibody at 1:250. Secondary Antibody: AlexaFluor 488. Counterstain: phalloidin (Alexa 647, red), beta tubulin (Anti-beta III Tubulin Ab, Alexa 555, magenta) Hoechst (blue). (A) Phalloidin (B) Anti-beta III Tubulin Ab. (C) NOS1/nNOS Antibody. (D) Hoechst (E) Composite.

Immunohistochemistry analysis using Mouse Anti-NOS1/ nNOS Monoclonal Antibody, Clone J23. Tissue: brain tissue. Species: Rat. Primary Antibody: Mouse Anti-NOS1/ nNOS Monoclonal Antibody at 1:100. Secondary Antibody: Goat anti-mouse IgG1: HRP at 1:100. Counterstain: DAPI at 1 ug/ml. A) 10X magnification. B) 20X magnification. C) Negative control mouse IgG (10 ug/ml), 20X magnification.

Western Blot analysis of Mouse Cerebellum showing detection of NOS1/ nNos protein using Mouse Anti-NOS1/ nNos Monoclonal Antibody, Clone J23. Lane 1: MW ladder. Lane 2: Mouse Brain Cerebellum (7.5 ug). Load: 7.5 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-NOS1/ nNos Monoclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.

Quick Database Links

Gene Symbol

NOS1

UniProt

P29475

NCBI Gene ID

4842

RefSeq (Protein):NP_000611.1