NOS1/ nNOS Antibody: HRP

• Catalog Number: orb699419
• Category: Antibodies
• Description: Mouse monoclonal antibody against NOS1 conjugated to HRP
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: J23
• Tested applications: ELISA, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG2a
• Immunogen: Partial Human Recombinant NOS1 Protein
• Concentration: 1mg/ml
• Dilution range: WB (1:1000)
• Conjugation: HRP
• MW: 160 kDa
• Target: NOS1
• Entrez: 4842
• UniProt ID: P29475
• NCBI: NP_000611.1
• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide and 0.23mM Citrate in dH2O
• Alternative names: BNOS antibody, Constitutive NOS antibody, IHPS 1 a
• Note: For research use only
• Application notes: A 1:1000 dilution of SMC-609 was sufficient for detection of NOS1/Nnos in 7.5 ug of Mouse Brain Cerebellum by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
• Expiration Date: 12 months from date of receipt.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-NOS1/ nNos Monoclonal Antibody, Clone J23. Tissue: Differentiated SH-SY5Y. Species: Human. Primary Antibody: Mouse Anti-NOS1/ nNos Monoclonal Antibody at 1:250. Secondary Antibody: AlexaFluor 488. Counterstain: phalloidin (Alexa 647, red), beta tubulin (Anti-beta III Tubulin Ab, Alexa 555, magenta) Hoechst (blue). (A) Phalloidin (B) Anti-beta III Tubulin Ab. (C) NOS1/nNOS Antibody. (D) Hoechst (E) Composite.

Immunohistochemistry analysis using Mouse Anti-NOS1/ nNOS Monoclonal Antibody, Clone J23. Tissue: brain tissue. Species: Rat. Primary Antibody: Mouse Anti-NOS1/ nNOS Monoclonal Antibody at 1:100. Secondary Antibody: Goat anti-mouse IgG1: HRP at 1:100. Counterstain: DAPI at 1 ug/ml. A) 10X magnification. B) 20X magnification. C) Negative control mouse IgG (10 ug/ml), 20X magnification.

Western Blot analysis of Mouse Cerebellum showing detection of NOS1/ nNos protein using Mouse Anti-NOS1/ nNos Monoclonal Antibody, Clone J23. Lane 1: MW ladder. Lane 2: Mouse Brain Cerebellum (7.5 ug). Load: 7.5 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-NOS1/ nNos Monoclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.