NOS1 Antibody

• Catalog Number: orb1260747
• Category: Antibodies
• Description: NOS1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human NOS1 (NP_000611.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IF: 1:20 - 1:50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 150kDa
• Target: NOS1
• UniProt ID: P29475
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: NOS1, nitric oxide synthase 1 (neuronal), NOS, neu
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IF: 1:20 - 1:50
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of Rat brain, using NOS1 antibody (orb1260747) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Immunofluorescence analysis of HeLa cells using NOS1 antibody (orb1260747). Blue: DAPI for nuclear staining.