NOS1 Antibody

• Catalog Number: orb1249514
• Category: Antibodies
• Description: NOS1 Antibody
• Target: NOS1
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Rat
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: C-ESKKDTDEVFSS
• UniProt ID: P29475
• Tested applications: ELISA, FC, IF, IHC, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.Immunohistochemistry:Cryosections of Human and Mouse Hypothalamus. Optimal dilution: 1:3000.Immunofluorescence: Expression of the protein seen in the nucleus and plasma membrane of HeLa cells and in the nucleus and cytoplasm of U2OS cells. Recommended concentration: 10ug/ml.Flow Cytometry: Flow cytometric analysis of Kelly cells. Recommended concentration: 10ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: NOS1, nitric oxide synthase 1 (neuronal), NOS, neu
• Research Area: Signal Transduction
• Note: For research use only
• NCBI: NP_001191142.1, NP_001191147.1, NP_000611.1

Immunostaining of NOS1 neurons in cryosection of a perfusion-fixed (4% PFA) mouse hypothalamus. HRP-staining with Ni-DAB, after Biotin-SP-anti-goat (IgG) method.

Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing nuclear and membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).