NOD1 Antibody (C-term)

• Catalog Number: orb1937939
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB29527
• Tested applications: IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:2000, WB: 1:1000, IHC-P-Leica: 1:500, IHC-P-Leica: 1:500
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 107691 Da
• Target: This NOD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 923-951 amino acids from the C-terminal region of human NOD1.
• UniProt ID: Q9Y239
• NCBI: NP_006083.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Nucleotide-binding oligomerization domain-containi
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-NOD1 Antibody (C-term) at 1:1000 dilution. Lane 1: CCRF-CEM whole cell lysate. Lane 2: NCI-H1299 whole cell lysate. Lane 3: HL-60 whole cell lysate. Lane 4: HT-29 whole cell lysate. Lane 5: SW480 whole cell lysate. Lane 6: Mouse heart lysate. Lane 7: Mouse spleen lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 108 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-NOD1 Antibody (C-term) at 1:2000 dilution. Lane 1: CCRF-CEM whole cell lysate. Lane 2: NCI-H1299 whole cell lysate. Lane 3: HL-60 whole cell lysate. Lane 4: HT-29 whole cell lysate. Lane 5: SW480 whole cell lysate. Lane 6: Mouse heart lysate. Lane 7: Mouse spleen lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 108 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis of paraffin-embedded human pancreas tissue performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary Antibody.

Immunohistochemical analysis of paraffin-embedded human spleen tissue performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary Antibody.