NOC4L Antibody

SKU: orb2636602

Description

Treg activation is critical for maintaining self-tolerance, but the translational control of this process is still poorly understood. Zhu et al. report a conserved ribosome biogenesis factor, Noc4L, that regulates mRNAs related to Treg and Tconv activation but does not affect global protein translation. Noc4L regulates translation of mRNAs related to Treg activation. Noc4L plays critical roles in activation of Tregs and Tconvs. Noc4L-mediated ribosome biogenesis is critical in controlling the activation of Tregs and maintaining immune tolerance. [Zhu et al., 2019, Cell Reports 27, 1205 1220.]

Images & Validation

• Tested Applications: FACS, IF
• Dilution Range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the NOC4L antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: PCRP-NOC4L-1B2
• Immunogen: Recombinant full-length human NOC4L protein was used as the immunogen for the NOC4L antibody.
• Purification: Protein A/G affinity
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

FACS staining of PFA-fixed human HeLa cells using NOC4L antibody (blue, clone PCRP-NOC4L-1B2) and isotype control (red).

Immunofluorescent staining of PFA-fixed human HeLa cells NOC4L antibody (green, clone PCRP-NOC4L-1B2) and phalloidin (red).

SDS-PAGE analysis of purified, BSA-free NOC4L antibody (clone PCRP-NOC4L-1B2) as confirmation of integrity and purity.

Quick Database Links

UniProt

Q9BVI4