NLRP3 Antibody (N-term)

• Catalog Number: orb1928431
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Target: This NLRP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 65-91 amino acids from the N-terminal region of human NLRP3.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: Q96P20
• MW: 118173 Da
• Tested applications: IHC-P, WB
• Dilution range: WB: 1:1000-1:2000, IHC-P: 1:10~50
• Antibody Type: Primary Antibody
• Clone Number: RB22404
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: NACHT, LRR and PYD domains-containing protein 3, A
• Research Area: Cancer Biology, Cardiovascular Research, Cell Biol
• Note: For research use only
• NCBI: NP_899632.1, NP_001073289.1, NP_001120934.1, NP_001120933.1, NP_001230062.1, NP_004886.3
• Expiration Date: 12 months from date of receipt.

NLRP3 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lymph tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the NLRP3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

All lanes: Anti-NLRP3 Antibody (N-term) at 1:1000-1:2000 dilution. Lane 1: Hela whole cell lysate. Lane 2: Jurkat whole cell lysate. Lane 3: Raji whole cell lysate. Lane 4: MDA-MB-231 whole cell lysate. Lane 5: THP-1 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 118 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.