NKX3.1 Antibody

SKU: orb2642864

Description

NKX3.1 is a prostate specific gene encoding a transcription factor that plays an important role in normal prostate development and carcinogenesis. It is a prostatic tumor suppressor gene located on chromosome 8p21.2, which frequently undergoes a loss of heterozygosity. NKX3.1 expression is highly restricted in prostate epithelial cells and therefore can be used as a diagnostic biomarker for prostate cancer and other metastatic lesions of prostatic origin. Furthermore, NKX3.1 shows better sensitivity than Prostate Specific Antigen (PSA) for identifying metastatic prostatic adenocarcinoma. This suggests that immunohistochemical staining of NKX3.1, along with other prostate-restricted markers, may be valuable for the definitive determination of prostatic origin in poorly differentiated metastatic carcinomas.

Images & Validation

• Tested Applications: IHC-P
• Dilution Range: The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Reactivity: Human
• Application Notes:
  Optimal dilution of the NKX3.1 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2c, kappa
• Clone No.: NKX3.1/2576
• Immunogen: A recombinant human NKX3.1 protein fragment within amino acids 92-224 was used as the immunogen for the NKX3.1 antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Prediluted in 1X PBS, 0.1 mg/ml rAlbumin, 0.05% sodium azide; For IHC use only
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IHC staining of FFPE human prostate carcinoma with NKX3.1 antibody (clone NKX3.1/2576). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

IHC staining of FFPE human prostate carcinoma with NKX3.1 antibody (clone NKX3.1/2576). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free NKX3.1 antibody (clone NKX3.1/2576) as confirmation of integrity and purity.

Quick Database Links

UniProt

Q99801