NKX2.8 Antibody

SKU: orb639676

Description

The protein encoded by this gene is a homeobox-containing developmental regulator associated with liver development. The encoded protein binds to the alpha-fetoprotein (AFP) gene promoter and increases the expression of AFP. This gene is overexpressed in some lung cancers and is linked to poor patient survival, possibly due to its resistance to cisplatin. This gene is aberrantly methylated in pancreatic cancer, deleted in squamous cell lung carcinomas, and acts as a tumor suppressor in esophageal cancer. Mutations in this gene may also be a cause of neural tube defects.

Images & Validation

• Tested Applications: ELISA, FACS, WB
• Dilution range: ELISA (order BSA-free format for coating),Flow cytometry: 1-2ug/million cells in 0.1ml,Western blot: 1-2ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: NKX28/2548
• Immunogen: A recombinant human partial protein (amino acids 10-123) was used as the immunogen for the NKX2.8 antibody.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

Western blot testing of human PC-3 and MCF-7 cell lysate with NKX2.8 antibody. Predicted molecular weight ~26 kDa, commonly observed at 26-34 kDa.

SDS-PAGE analysis of purified, BSA-free NKX2.8 antibody as confirmation of integrity and purity.

Quick Database Links

UniProt

O15522