Recombinant NKX2.2 Antibody / Rabbit Monoclonal

SKU: orb322972

Description

NKX2.2 acts as a transcriptional activator. Required for the maintenance of NEUROD1 expression in the horomone-producing endocrine cells of the pancreas. May be involved in specifying diencephalic neuromeric boundaries, and in controlling the expression of genes that play a role in axonal guidance. Associates with chromatin at the NEUROD1 promoter region. Binds to a subset of consensus elements within the NEUROD1 promoter (By similarity). [UniProt]

Images & Validation

• Tested Applications: IHC-P
• Dilution Range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)
• Reactivity: Human
• Application Notes:
  Titering of the recombinant NKX2.2 antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: Rabbit IgG, kappa
• Clone No.: RMNK2-1
• Immunogen: A human partial recombinant protein was used as the immunogen for this recombinant NKX2.2 antibody.
• Purification: Protein A affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IHC testing of FFPE human pancreas with recombinant NKX2.2 antibody (clone RMNK2-1). HIER: steam sections in pH9 10 mM Tris with 1 mM EDTA for 10-20 min.

IHC testing of FFPE Ewings sarcoma with recombinant NKX2.2 antibody (clone RMNK2-1). HIER: steam sections in pH9 10 mM Tris with 1 mM EDTA for 10-20 min.