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NKX2-4 Antibody

SKU: orb1539215

Description

NKX2-4 Antibody

Images & Validation

Tested ApplicationsELISA, ICC, IF, IHC, IHC-P, WB
Dilution RangeELISA, ICC, IF, IHC, IHC-P (5 µg/ml), WB (1 - 2 µg/ml)
ReactivityHuman, Mouse
Application Notes
Further information: NKX2-4 antibody can be used for detection of NKX2-4 by Western blot at 1 ug/mL. Antibody can also be used for immunocytochemistry starting at 2.5 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Western Blot:

Key Properties

HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenAn 18 AA synthetic peptide near the center of human NKX2-4. The immunogen is located within aa 220-270 of NKX2-4.
TargetNKX2-4
PurificationPeptide Column Affinity Chromatography
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesPBS containing 0.02% Sodium Azide
Concentration1 mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

NKX2-4, Homeobox protein Nkx-2.4, NKX2.4, NKX2D, NK-2 (Drosophila) homolog D, NK-2 homolog D, NK2 homeobox 4, Nkx-2.4, NKX24, Tinman

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NKX2-4 Antibody

Immunocytochemistry of NKX2-4 in A20 cells with NKX2-4 antibody at 2.5 ug/ml.

NKX2-4 Antibody

Immunofluorescence of NKX2-4 in A20 cells with NKX2-4 antibody at 20 ug/ml.

NKX2-4 Antibody

Western blot analysis of NKX2-4 in A20 cell lysate with NKX2-4 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol
IF
Immunofluorescence
View Protocol
ICC
Immunocytochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

NKX2-4 Antibody (orb1539215)

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Available Sizes

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0.05 mg
$ 740.00
DispatchUsually dispatched within 2-3 weeks
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