NKCC2 Antibody (APC)

SKU: orb152682

Description

Rabbit polyclonal to NKCC2 (APC). NKCC2, a kidney-specific member of the cation chloride co-transporter family SLC12, facilitates transport of sodium, potassium, and chloride ions from the lumen of the renal thick ascending limb into the cell. Transport of sodium dilutes the luminal fluid, decreasing its osmolality creating an osmotic driving force for water reabsorption in the connecting tubule and cortical collecting duct under the influence of the hormone vasopressin. NKCC2 is found on the apical surface of the thick ascending limb of the loop of Henle and is blocked by loop diuretics such as furosemide..

Images & Validation

• Tested Applications: IHC, WB
• Dilution range: WB (1:1000); IHC (1:25)
• Reactivity: Human, Mouse, Rat
• Application Notes:
  1 µg/ml of SPC-401 was sufficient for detection of NKCC2 in 10 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Produced against a synthetic peptide mapping to a segment (amino acids 33-55) of the N-terminal tail of rat NKCC2 (antibody designation L224).
• Target: NKCC2
• Molecular Weight: 160kDa
• Purification: Protein A Purified

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 95.46mM Phosphate, 2.48mM MES and 2mM EDTA
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

SLC12A1, sodium potassium chloride cotransporter 2, sodium-potassium-chloride cotransporter isoform 2, BSC1, MBSC1, Na-K-2Cl cotransporter, NKCC2A variant A, RBSC

Immunohistochemistry analysis using Rabbit Anti-NKCC2 Polyclonal Antibody. Tissue: Kidney. Species: Rat. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-NKCC2 Polyclonal Antibody at 1:25 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit IgG: Alexa Fluor 488. Counterstain: Actin-binding Phalloidin-Alexa Fluor 633; DAPI (blue) nuclear stain. Magnification: 63X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) NKCC2 Antibody (D) Composite

Western blot analysis of Rat kidney tissue lysates showing detection of NKCC2 protein using Rabbit Anti-NKCC2 Polyclonal Antibody. Primary Antibody: Rabbit Anti-NKCC2 Polyclonal Antibody at 1:1000.

Quick Database Links

Gene Symbol

NKCC2

UniProt

Q5PQV1

NCBI Gene ID

25065

RefSeq (Protein):NP_001257546.1