NK1.1 mAb (PK136),InVivoPure

SKU: orb3127224

Description

The PK136 monoclonal antibody is a widely used mouse IgG2a antibody that specifically recognizes NK1.1, a surface glycoprotein belonging to the NKR-P1 (CD161) family. This Marker is expressed on NK cells and NKT cells in certain mouse strains including C57BL/6, FVB/N and NZB. Due to its specificity, PK136 is extensively used in immunological research to study NK cell biology, innate immunity, and tumor immunology. The PK136 antibody binds to NKR-P1C (NK1.1) on NK cells and a subset of T cells known as NKT cells in NK1.1-expressing strains. It does not cross-react with NK cells from BALB/c or other NK1.1-negative strains. In addition to Flow and IHC, this clone has also been reported to work for in-vitro and in-vivo depletion assays.

Images & Validation

• Reactivity: Mouse
• Application Notes:
  Recomended Isotype: Mouse IgG2A k Isotype Control, Recomended Isotype Catalog: orb2568794

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: m IgG2a
• Clone No.: PK136
• Immunogen: Spleen enriched for NK1+ cells and bone marrow cells from CE mice
• Target: NK1.1
• Purity: ≥ 90 % (CGE, reducing conditions) ≤ 10 % aggregates (analytical SEC) Endotoxin level ≤ 2 EU/mg
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Clear Liquid, PBS, pH 7.4, 0.2 μm sterile filtered
• Concentration: ≥ 1 mg/ mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CD161c, Killer cell lectin-like receptor subfamily B member 1C, CD161 antigen-like family member C, Lymphocyte antigen 55c, Ly-55c, NK1.1, NKR-P1.9, NKR-P1C, Natural killer cell surface protein P1-40, NKR-P1 40, PK136,

Quick Database Links

Gene Symbol

NK1.1

UniProt

P27814