Nitrotyrosine Antibody (FITC)

SKU: orb147399

Description

Mouse monoclonal to Nitrotyrosine (FITC). Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxide signaling. While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosino-philperoxidase, myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. Nitrotyrosine may also serve as a biomarker for the effects of reactive nitrogen oxides, based on tyrosine residues becoming nitrated in proteins at sites of inflammation induced tissue injury. The presence of nitro tyrosine-containing proteins therefore has shown high correlation to disease states such as atherosclerosis, Alzheimers disease, Parkinsons disease and amyotrophic lateral sclerosis..

Research Area

Cancer Biology, Cell Biology, Neuroscience, Protein Biochemistry, Signal Transduction

Images & Validation

• Tested Applications: AM, ELISA, FC, ICC, IF, IHC, IP, WB
• Dilution Range: WB (1:1400), IHC (1:100)
• Reactivity: All
• Application Notes:
  0.7 µg/ml was sufficient for detection of 5 µg SIN-1 treated BSA by Western Blot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone No.: 39B6
• Immunogen: 3-(4-hydroxy-3-nitrophenylacetamido) propionic acid-bovine serum albumin
• Target: Nitrotyrosine
• Purification: Protein G Purified
• Conjugation: FITC

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 640.91mM DMSO, 136.36 mM Ethanolamine, 126.89 mM chlorides, 9.09mM phosphates, 9.09mM NaHCO3
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Nitrotyrosine, Nitro tyrosine, 3-Nitrotyrosine

Quick Database Links

Gene Symbol

Nitrotyrosine