Nitro blue tetrazolium chloride

SKU: orb1218203

Description

Nitro blue tetrazolium chloride is a NADPH-diaphorase substrate that competitively inhibits nitric oxide synthase (IC50 = 3-4 M). A well-known scavenger of superoxide anions.Nitro blue tetrazolium chloride, a substrate for dehydrogenases, is used with the alkaline phosphatase substrate BCIP in western blotting and immunohistological staining procedures.

Key Properties

• CAS Number: 298-83-9
• MW: 817.64
• Purity: >98% (HPLC)
• Formula: C₄₀H₃₀Cl₂N₁₀O₆
• SMILES: COC1=CC(C2=CC=C(N3N=C(C4=CC=CC=C4)N=[N+]3C5=CC=C([N+]([O-])=O)C=C5)C(OC)=C2)=CC=C1N6N=C(C7=CC=CC=C7)N=[N+]6C8=CC=C([N+]([O-])=O)C=C8.[Cl-].[Cl-]
• Target: NOS
• Solubility: 70%DMF:100 mg/mL (122.30 mM);DMSO:33.33 mg/mL (40.76 mM; Need ultrasonic)

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NBT

Quick Database Links

Gene Symbol

NOS