BCIP/NBT 1-Component AP Membrane Substrate (SUBB)

SKU: orb511048

Description

BCIP/NBT 1-Component AP Membrane Substrate (SUBB) is a ready-to-use formulation containing BCIP(5-bromo-4-Chloro-3′-Indolyphosphate p-Toluidine Salt) and NBT (Nitro-Blue Tetrazolium Chloride) in a mildly alkaline buffer. SUBB is useful for immunoblotting applications where alkaline phosphatase-conjugated molecules are used for detection. NBT serves as the oxidant and BCIP is the AP substrate. SUBB reacts with AP yielding a dark blue reaction product.

Images & Validation

• Application Notes:
  1. Allow product to equilibrate to room temperature (25°C) prior to use., 2. Add stop solution to plate well. Equal volumes of TMB microwell substrate and stop solution should be used. We recommend using 100 µL of TMB substrate and 100 µL of Stop Solution for TMB Substrates per well. The stopped TMB reaction product is stable for 1 hour., 3. Read absorbance at 450 nm within 60 minutes. For best results, sample absorbance should be monitored and stopped before values exceed 2.0 OD units.

Storage & Handling

• Storage: 2-8°C
• Concentration: 1 X
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only