Nicastrin Antibody

• Catalog Number: orb1238794
• Category: Antibodies
• Description: Nicastrin Antibody
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Nicastrin antibody was raised against a 18 amino acid synthetic peptide from near the center of human Nicastrin.The immunogen is located within amino acids 370 - 420 of Nicastrin.
• Antibody Type: Primary Antibody
• Concentration: 1 mg/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: NCSTN
• UniProt ID: Q92542
• NCBI: NP_056146
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Nicastrin Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: Nicastrin Antibody: ATAG1874, KIAA0253, UNQ1874/PR
• Note: For research use only
• Application notes: Nicastrin antibody can be used for detection of Nicastrin by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Nicastrin in human brain tissue lysate with Nicastrin antibody at (A) 0.5 and (B) 1 µg/mL.

Immunohistochemistry of Nicastrin in human brain tissue with Nicastrin antibody at 2.5 µg/mL.

Immunofluorescence of Nicastrin in Human Brain cells with Nicastrin antibody at 20 µg/mL.

Immunofluorescence of Nicastrin in mouse brain tissue with Nicastrin antibody at 20 µg/ml. Green: Nicastrin Antibody (orb1238794) Blue: DAPI staining.