NHE3 Antibody: HRP

• Catalog Number: orb152668
• Category: Antibodies
• Description: Rabbit polyclonal to NHE3 (HRP). Sodium-hydrogen exchanger 3 (NHE3; Slc9a3) is an epithelial transport protein that carries out 1:1 exchange of Na+ and H+ across the plasma membrane. It is apically located in the proximal tubule of the kidney, the thick ascending limb of the kidney, and in small intestine. NHE3 is phosphorylated and regulated by multiple kinases including PKA, SGK1 and CK2. It can be phosphorylated by calyculin A, and dephosphorylated by PP1 catalytic subunit in vitro..
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Mouse, Rat
• Immunogen: Synthetic peptide mapping to AA 809 to 831 of rat sequence
• Concentration: 1 mg/ml
• Dilution range: WB (1:1000); IHC (1:25)
• Conjugation: HRP
• MW: 84kDa
• Target: NHE3
• Entrez: 24784
• UniProt ID: P26433
• NCBI: NP_036786
• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide and 0.23mM Citrate in dH2O
• Alternative names: Sodium / Hydrogen Exchanger 3 antibody, SLC9A3 ant
• Note: For research use only
• Application notes: 1 µg/ml of SPC-400 was sufficient for detection of HNE3 in 10 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
• Expiration Date: 12 months from date of receipt.

Immunohistochemistry analysis using Rabbit Anti-NHE3 Polyclonal Antibody. Tissue: Colon. Species: Rat. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-NHE3 Polyclonal Antibody at 1:25 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit IgG:Alexa Fluor 488. Counterstain: Actin-binding Phalloidin-Alexa Fluor 633; DAPI (blue) nuclear stain. Magnification: 63X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) NHE3 Antibody (D) Composite

Western blot analysis of Rat kidney tissue lysates showing detection of NHE3 protein using Rabbit Anti-NHE3 Polyclonal Antibody. Primary Antibody: Rabbit Anti-NHE3 Polyclonal Antibody at 1:1000.