NHE3 Antibody (Biotin)

SKU: orb152666

Description

Rabbit polyclonal to NHE3 (Biotin). Sodium-hydrogen exchanger 3 (NHE3; Slc9a3) is an epithelial transport protein that carries out 1:1 exchange of Na+ and H+ across the plasma membrane. It is apically located in the proximal tubule of the kidney, the thick ascending limb of the kidney, and in small intestine. NHE3 is phosphorylated and regulated by multiple kinases including PKA, SGK1 and CK2. It can be phosphorylated by calyculin A, and dephosphorylated by PP1 catalytic subunit in vitro..

Research Area

Cancer Biology, Neuroscience, Pharmacology & Drug Discovery, Signal Transduction

Images & Validation

• Tested Applications: ICC, IF, IHC, WB
• Dilution Range: WB (1:1000); IHC (1:25)
• Reactivity: Mouse, Rat
• Application Notes:
  1 µg/ml was sufficient for detection of HNE3 in 10 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide mapping to AA 809 to 831 of rat sequence
• Target: NHE3
• Molecular Weight: 84kDa
• Purification: Protein A Purified
• Conjugation: Biotin

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Sodium / Hydrogen Exchanger 3, SLC9A3, Na+/H+ exchanger 3, isoform 3, Solute carrier family 9 member 3, NHE3MGC126720, MGC126718, solute carrier family 9 (sodium/hydrogen exchanger) member 3, NHE-3, Na(+)/H(+) exchanger 3, Sodium/hydrogen exchanger 3

Quick Database Links

Gene Symbol

NHE3

UniProt

P26433

NCBI Gene ID

24784

RefSeq (Protein):NP_036786