NHE3 Antibody (APC)

SKU: orb152665

Description

Rabbit polyclonal to NHE3 (APC). Sodium-hydrogen exchanger 3 (NHE3; Slc9a3) is an epithelial transport protein that carries out 1:1 exchange of Na+ and H+ across the plasma membrane. It is apically located in the proximal tubule of the kidney, the thick ascending limb of the kidney, and in small intestine. NHE3 is phosphorylated and regulated by multiple kinases including PKA, SGK1 and CK2. It can be phosphorylated by calyculin A, and dephosphorylated by PP1 catalytic subunit in vitro..

Research Area

Cancer Biology, Neuroscience, Pharmacology & Drug Discovery, Signal Transduction

Images & Validation

• Tested Applications: ICC, IF, IHC, WB
• Dilution range: WB (1:1000); IHC (1:25)
• Reactivity: Mouse, Rat
• Application Notes:
  1 µg/ml of SPC-400 was sufficient for detection of HNE3 in 10 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide mapping to AA 809 to 831 of rat sequence
• Target: NHE3
• Molecular Weight: 84kDa
• Purification: Protein A Purified
• Conjugation: APC

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 95.46mM Phosphate, 2.48mM MES and 2mM EDTA
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Sodium / Hydrogen Exchanger 3, SLC9A3, Na+/H+ exchanger 3, isoform 3, Solute carrier family 9 member 3, NHE3MGC126720, MGC126718, solute carrier family 9 (sodium/hydrogen exchanger) member 3, NHE-3, Na(+)/H(+) exchanger 3, Sodium/hydrogen exchanger 3

Immunohistochemistry analysis using Rabbit Anti-NHE3 Polyclonal Antibody. Tissue: Colon. Species: Rat. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-NHE3 Polyclonal Antibody at 1:25 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit IgG:Alexa Fluor 488. Counterstain: Actin-binding Phalloidin-Alexa Fluor 633; DAPI (blue) nuclear stain. Magnification: 63X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) NHE3 Antibody (D) Composite

Western blot analysis of Rat kidney tissue lysates showing detection of NHE3 protein using Rabbit Anti-NHE3 Polyclonal Antibody. Primary Antibody: Rabbit Anti-NHE3 Polyclonal Antibody at 1:1000.

Quick Database Links

Gene Symbol

NHE3

UniProt

P26433

NCBI Gene ID

24784

RefSeq (Protein):NP_036786