NGI-1

SKU: orb1220427

Description

NGI-1 is a a cell-permeable inhibitor of oligosaccharyltransferase (OST).can effectively reduce virus infectivity without affecting cell viability.

Key Properties

• CAS Number: 790702-57-7
• MW: 394.51
• Purity: >98% (HPLC)
• Formula: C₁₇H₂₂N₄O₃S₂
• SMILES: CN(C)S(=O)(=O)c1ccc(N2CCCC2)c(c1)C(=O)Nc1ncc(C)s1
• Target: oligosaccharyltransferase
• Solubility: DMSO:160 mg/mL (405.57 mM)

Bioactivity

• In Vitro:
  NGI-1 inhibits the glycosylation of LASV GP mediated by STT3A-OST (in STT3B- and MAGT1-TUSC3- cells) or STT3B-OST (in STT3A- cells) and impaires its proteolytic cleavage in a dose-dependent manner. NGI-1 blocks EGFR N-linked glycosylation in lung adenocarcinoma cells as assessed. In controls EGFR is biotinylated, consistent with its plasma membrane expression, but in NGI-1 treated cells the EGFR is predominantly found in the non-biotinylated intracellular fraction suggesting a change in cellular localization. Western blot analysis. Cell line: STT3A-, STT3B- and MAGT1-TUSC3- cells. Concentration: 1, 2, 5 μM. Incubation time: 36 hours. Result: Inhibited the glycosylation of LASV GP mediated by STT3A-OST (in STT3B- and MAGT1-TUSC3- cells) or STT3B-OST (in STT3A- cells) and impaired its proteolytic cleavage in a dose-dependent manner.
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Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ML414

Quick Database Links

Gene Symbol

oligosaccharyltransferase