NGFR/p75 neurotrophin receptor (p75NTR) Antibody

SKU: orb3167470

Description

Our Anti-NGFR/p75 neurotrophin receptor (p75NTR) mouse monoclonal primary antibody detects human, mouse, and rat NGFR/p75 neurotrophin receptor (p75NTR), and is IgG. It is validated for use in FC, ICC, IHC-Frozen, Immunopanning, IP, WB.

Images & Validation

• Tested Applications: FC, ICC, IHC, IP, WB
• Dilution Range: WB: 0.5-2.0 µg/mL, non-reducing conditions only (no DTT or beta-mercaptoethanol).; ICC: 1-5 µg/mL; IP: Lysate dependent. 10 ug per 200-500 ug total protein
• Reactivity: Human, Mouse, Rat
• Application Notes:
  Flow Cytometry: 5-20 µg/mL. Immunopanning: 1-5 µg/mL. ICC: Staining is strongest in non-fixed cells, light fixation is tolerable. IHC: fresh, acetone fixed sections only, epitope is fixation sensitive. Not suitable in formalin-fixed, paraffin (FFPE) embedded tissues.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone No.: 8J2
• Immunogen: Recombinant extracellular domain (amino acids 29-250) of human NGFR/p75NTR protein with N-terminal His-tag.
• Target: NGFR/p75 neurotrophin receptor (p75NTR)
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Lyophilized from a solution containing PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Low-affinity nerve growth factor receptor; NGF receptor; Gp80-LNGFR; p75 ICD; Low affinity neurotrophin receptor p75NTR; CD271

Quick Database Links

Gene Symbol

NGFR/p75 neurotrophin receptor (p75NTR)

UniProt

P08138