NFKBIA Antibody ( S32/36 )

SKU: orb38812

This product can be custom made

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Description

Peptide affinity purified rabbit polyclonal antibody.

Research Area

Cancer Biology, Cell Biology, Immunology & Inflammation, Immunology & inflammation, Infectious Disease & Virology, Signal Transduction

Images & Validation

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Item 1 of 4

Tested Applications	FC, IF, IHC-P, WB
Dilution Range	IF - 1:10-50, WB - 1:1000, IHC-P - 1:50-100, FC - 1:10-50
Reactivity	Human
Predicted Reactivity	Mouse, Porcine

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Immunogen	Synthetic Peptide
Target	NFKBIA
Molecular Weight	35609
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Anti-NF-kappa-B inhibitor alpha antibody, anti-I-kappa-B-alpha antibody, anti-IkappaBalpha antibody, anti-IkB-alpha antibody, anti-Major histocompatibility complex enhancer-binding protein MAD3 antibody, anti-NFKBIA antibody, anti-IKBA antibody, anti-MAD3 antibody, anti-NFKBI antibody

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Western blot analysis of NFKBIA-S32/36 in Hela cell line lysates (35 ug/lane). NFKBIA (arrow) was detected using the purified Pab.

Confocal immunofluorescent analysis of NFKBIA Antibody (S32/36) with ZR-75-1 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).

Flow cytometric analysis of hela cells using NFKBIA Antibody (S32/36) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human hepatocarcinoma with NFKBIA Antibody (S32/36), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.