NFKBIA Antibody ( S32/36 )

SKU: orb38812

Description

Peptide affinity purified rabbit polyclonal antibody.

Research Area

Cancer Biology, Cell Biology, Immunology & Inflammation, Immunology & inflammation, Infectious Disease & Virology, Signal Transduction

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Dilution Range: WB: 1:1000, IHC-P: 1:50-100, FACS: 1:10-50, IF/ICC: 1:10-50
• Reactivity: Human
• Predicted Reactivity: Mouse, Porcine

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB21911
• Immunogen: Synthetic Peptide
• Target: NFKBIA
• Molecular Weight: 35609
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-NF-kappa-B inhibitor alpha antibody, anti-I-kappa-B-alpha antibody, anti-IkappaBalpha antibody, anti-IkB-alpha antibody, anti-Major histocompatibility complex enhancer-binding protein MAD3 antibody, anti-NFKBIA antibody, anti-IKBA antibody, anti-MAD3 antibody, anti-NFKBI antibody

Western blot analysis of NFKBIA-S32/36 in Hela cell line lysates (35 ug/lane). NFKBIA (arrow) was detected using the purified Pab.

Confocal immunofluorescent analysis of NFKBIA Antibody (S32/36) with ZR-75-1 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).

Flow cytometric analysis of hela cells using NFKBIA Antibody (S32/36) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human hepatocarcinoma with NFKBIA Antibody (S32/36), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Quick Database Links

Gene Symbol

NFKBIA

UniProt

P25963

RefSeq (Protein):NP_065390.1