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Catalog Number | orb1261293 |
---|---|
Category | Antibodies |
Description | NFKB1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, IP, WB |
Reactivity | Human |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 740-964 of human NFKB1 (NP_001158884.1). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:1000IHC: 1:50 - 1:200IF: 1:20 - 1:100IP: 1:20 - 1:50 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 120kDa |
Target | NFKB1 |
UniProt ID | P19838 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | NFKB1, p50, KBF1, p105, EBP-1, NF-kB1, NFKB-p50, N Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:1000IHC: 1:50 - 1:200IF: 1:20 - 1:100IP: 1:20 - 1:50 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using NFKB1 antibody (orb1261293) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human tonsil using NFKB1 antibody (orb1261293) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using NFKB1 antibody (orb1261293) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF7 cells using NFKB1 antibody (orb1261293).
Immunoprecipitation analysis of 150 ug extracts of MCF-7 cells using 3 ug NFKB1 antibody (orb1261293). Western blot was performed from the immunoprecipitate using NFKB1 antibody (orb1261293) at a dilution of 1:500.
ChIP, ELISA, IF, IHC, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IF, IHC-P, WB | |
Guinea pig, Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC-P, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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