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NFKB1 Antibody
SKU: orb1261293
Featured
Description
Research Area
Cancer Biology, Cell Biology, Epigenetics & Chromatin, Immunology & Inflammation, Signal Transduction
Images & Validation
−Item 1 of 5
| Tested Applications | IF, IHC, IP, WB |
|---|---|
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 740-964 of human NFKB1 (NP_001158884.1). |
| Target | NFKB1 |
| Molecular Weight | Observed: 120kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−NFKB1, p50, KBF1, p105, EBP-1, NF-kB1, NFKB-p50, NFkappaB, NF-kappaB, NFKB-p105, NF-kappa-B
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Western blot analysis of extracts of various cell lines, using NFKB1 antibody (orb1261293) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human tonsil using NFKB1 antibody (orb1261293) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using NFKB1 antibody (orb1261293) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF7 cells using NFKB1 antibody (orb1261293).
Immunoprecipitation analysis of 150 ug extracts of MCF-7 cells using 3 ug NFKB1 antibody (orb1261293). Western blot was performed from the immunoprecipitate using NFKB1 antibody (orb1261293) at a dilution of 1:500.
Quick Database Links
Gene Symbol
NFKB1
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
IP
Immunoprecipitation
NFKB1 Antibody (orb1261293)
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