RELA Antibody

SKU: orb1253831

Description

RELA Antibody

Research Area

Cancer Biology, Cell Biology

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: NFkB-p65 (Phospho-Ser536) antibody was raised against a peptide sequence around phosphorylation site of serine 536 (F-S-S (p) -I-A) derived from Human NFκB-p65.
• Target: RELA
• Molecular Weight: 65 kDa
• Purification: Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

p65, NFKB3, PKB, RAC, RELA, TF65, Transcription factor p65, p65

Western blot analysis of lysed extracts from HepG2 and HL60 cells untreated or treated with TNF using NFλB-p65 (Phospho-Ser536).

Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NFλB-p65 (Phospho-Ser536). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using NFλB-p65 (Phospho-Ser536).

Quick Database Links

Gene Symbol

RELA

UniProt

Q04206

RefSeq (Protein):NP_001138610.1