RELA Antibody

SKU: orb345375

Description

NFkB p65 (phospho-S276) antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:5,000 - 1:25,000, WB: 1:1,000
• Reactivity: Human
• Application Notes:
  Anti-NFKB phospho specific polyclonal antibody reacts with human pS276 p65 and shows minimal reactivity when tested by western blot with non-phosphorylated p65 and minimal reactivity when tested by ELISA against the non-phosphorylated form of the immunizing peptide. For immunoblotting a 1:1,000 dilution is recommended. A 65 kDa band corresponding to human p65 is detected. HeLa cells or TNF inducible KBM-5 cells can be used as a positive control. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: NFkB p65 (Rel A) peptide corresponding to an internal region near phospho Serine 276 of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
• Target: RELA
• Purity: Rabbit Anti-NFKB p65 (RelA) pS276 Antibody was prepared from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. This phospho specific polyclonal antibody is specific for phosphorylated pS276 human p65. Reactivity with non-phosphorylated pS267 is minimal. Cross reactivity with pS276 phosphorylated p65 from mouse, rat or other species has not been determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-NFKB p65 pS276 antibody, rabbit anti-p65 antibody, rabbit anti-RelA antibody, NFKB, NFkß, NF-kB, NF-kappaB, NFkappaB, Transcription factor p65, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, RELA, NFKB3

Quick Database Links

Gene Symbol

RELA

UniProt

Q04206

RefSeq:223468676