NFkB p65 Mouse mAb

• Catalog Number: orb763457
• Category: Antibodies
• Description: Mouse monoclonal antibody to NFkB p65
• Clonality: Monoclonal
• Isotype: IgG1
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1mg/mL
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
• Immunogen: Recombinant Protein
• UniProt ID: Q04206
• MW: 65kDa
• Tested applications: IF, IHC, IP, WB
• Dilution range: WB 1:500-2,000 IP 1:200 IF 1:100-200 IHC 1:200-500
• Source: Mouse
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-NFKB3 antibody, anti-p65 antibody, anti-RELA
• Note: For research use only
• Entrez: 5970

Western blot analysis of extracts from HeLa (Lane 1), MCF-7 (Lane 2), Mouse Liver (Lane 3), Rat Kidney (Lane 4), using NFκB p65 diluted at 1:1, 500.

1: Input: Hela Cell Lysate 2: IP product: IP dilute 1:200 Western blot analysis: primary antibody : orb763457 1:2, 000 Secondary antibody: Goat anti-Mouse IgG, Light chain specific (S003), 1:5, 000.

IF analysis of Hela with orb763457 (Left) and DAPI (Right) diluted at 1:100.

Immunohistochemical analysis of paraffin-embedded Rat Brain Tissue using NFkB p65 (orb763457) Mouse mAbdiluted at 1:500.