NFkB p65 Monoclonal Antibody(5G6)

• Catalog Number: orb1415915
• Category: Antibodies
• Description: Mouse monoclonal antibody to NFkB p65.
• Clonality: Monoclonal
• Species/Host: Mouse
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• UniProt ID: Q04206
• Tested applications: IF, IHC-P, IP, WB
• Dilution range: WB: WB: 1:500-2000 IP:1:200 IF 1:200 IHC 1:50-300, IHC: 1:50-300, IF: 1:200, WB: 1:500-2000, IP: 1:200
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: RELA; NFKB3; Transcription factor p65; Nuclear fac
• Note: For research use only

1) Input: Hela Cell Lysate 2) IP product: IP dilute 1: 200

Western blot analysis of 1) Hela, 2) Rat Heart Tissue, 3) Mouse Spleen Tissue, diluted at 1: 2000.

Immunofluorescence analysis of Human-appendix tissue. 1, NFkB p65 Monoclonal Antibody (5G6) (red) was diluted at 1: 200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1: 300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B

Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1, NFkB p65 Monoclonal Antibody (5G6) was diluted at 1: 200 (4°C, overnight). 2, Sodium citrate pH6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1: 200 (room tempeRature, 30 min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Human-Appendix tissue. 1, NFkB p65 Monoclonal Antibody (5G6) was diluted at 1: 200 (4°C, overnight). 2, Sodium citrate pH6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1: 200 (room tempeRature, 30 min). Negative control was used by secondary antibody only.