RELA Antibody

SKU: orb750588

Description

NFkB p65 antibody

Images & Validation

• Tested Applications: ChIP, ELISA, Gel Supershift Assays, IHC, WB
• Dilution Range: ELISA: 1:5,000, ChIP: 1 μl/IP, Gel Supershift Assays: 05 µL - 1.0 µL, IHC: 1:400, WB: 1:2,000 - 1:5,000
• Reactivity: Human, Mouse
• Application Notes:
  Anti-NFkB p65 (Rel A) is tested by immunoblot of human and mouse NFkB p65 (Rel A), immunohistochemistry, and ELISA. This product was also tested in a gel supershift assay, IP, IF, and ChIP and found to be reactive against all p65 (Rel A) containing human, mouse or rat NFkB complexes using 0.5 to 1.0 µl per assay.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Antiserum
• Immunogen: NFkB p65 (Rel A) peptide corresponding to a region near the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
• Target: RELA
• Purity: This product was prepared from monospecific antiserum by delipidation and defibrination. Anti-NFkB p65 (Rel A) may react non-specifically with other proteins. Control peptide (code #100-4165p) will compete only with the specific reaction of antiserum with the NFkB p65 (Rel A) subunit.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 80 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit Anti-NFKB p65 antibody, rabbit Anti-Rel A antibody, NFKB, nfkb, NF-kB, NF-kappaB, NFkappaB, Nuclear factor NF-kappa-B p65 subunit

Immunohistochemistry of NFKB p65 (Rel A) antibody. Tissue: lymphocytes and germinal center cells of the human tonsil. Fixation: formalin fixed paraffin embedded. Antigen retrieval: user optimized. Primary antibody: NFKB p65 (Rel A) antibody at 1:400. Secondary antibody: Peroxidase goat anti-rabbit at (p/n orb347654) 1:10000 for 45 min at RT. Localization: nuclear and occasionally cytoplasmic. Staining: Moderate positive nuclear or cytoplasmic staining was observed in lymphocytes and germinal center cells of the tonsil.

Western Blot of Rabbit anti-NFkB antibody. Marker: Opal Pre-stained ladder. Lane 1: HEK293 lysate (p/n orb348669). Lane 2: HeLa Lysate (p/n orb348668). Lane 3: MCF-7 Lysate (p/n orb348664). Lane 4: Jurkat Lysate. Lane 5: A431 Lysate (p/n orb348665). Lane 6: A549 Lysate (p/n orb348675). Lane 7: LNCap Lysate (p/n orb348694). Lane 8: MOLT-4 Lysate (p/n orb348696). Lane 9: Ramos Lysate. Lane 10: Raji Lysate (p/n orb348672). Lane 11: A-172 Lysate (p/n orb348708). Lane 12: NIH/3T3 Lysate (p/n orb348714). Load: 10 µg per lane. Primary antibody: NFkB antibody at 1:500 overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody (p/n orb347654) at 1:30000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS for 30 min at RT. Predicted/Observed size: 65 kDa for NFkB.

Western Blot of Rabbit anti-NFkB p65 (Rel A) antibody. Lane 1: HeLa cell lysate (p/n orb348668). Lane 2: HeLa cell lysate (p/n orb348668). Load: 35 µg per lane. Primary antibody: NFkB p65 Rel A antibody at 1:5000 for 2 H at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:2000 for 60 min at RT. Block: 5% BLOTTO 2 H at RT. Predicted/Observed size: ~65 kDa, ~65 kDa for NFkb p65 Rel A. Other band(s): None.

Quick Database Links

Gene Symbol

RELA

UniProt

Q04206

RefSeq:223468676