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NFE2L1 Antibody
SKU: orb1259805
Featured
Description
Images & Validation
−Item 1 of 10
| Tested Applications | IF, IHC, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 515-772 of human NFE2L1 (NP_003195.1). |
| Target | NFE2L1 |
| Molecular Weight | Observed: 64kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Liquid |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−NFE2L1, FLJ00380, LCR-F1, NRF1, TCF11
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Western blot analysis of extracts of various cell lines, using NFE2L1 antibody (orb1259805) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry of paraffin-embedded rat spleen using NFE2L1 antibody (orb1259805) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using NFE2L1 antibody (orb1259805) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using NFE2L1 antibody (orb1259805) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using NFE2L1 antibody (orb1259805) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human placenta using NFE2L1 antibody (orb1259805) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using NFE2L1 antibody (orb1259805) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human uterus using NFE2L1 antibody (orb1259805) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using NFE2L1 antibody (orb1259805) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U-2 OS cells using CCDC59 antibody (orb1259969) at dilution of 1:100. Blue: DAPI for nuclear staining.
Quick Database Links
Gene Symbol
NFE2L1
UniProt
UniProt Details
− No UniProt data available
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
NFE2L1 Antibody (orb1259805)
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