NFκB-p65 (Acetyl Lys310) rabbit pAb Antibody

SKU: orb763969

Description

Rabbit polyclonal antibody to NF kappa B-p65 (Acetyl Lys310)

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Dilution Range: WB 1:500-2000, IHC-p 1:50-300, IF 1:50-300
• Reactivity: Human, Mouse

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human NF-kappaB p65 around the acetylated site of Lys310. AA range:275-324
• Molecular Weight: 65
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-RELA antibody, anti-NFKB3 antibody, anti-Transcription factor p65 antibody, anti-Nuclear factor NF-kappa-B p65 subunit antibody, anti-Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 antibody

Immunofluorescence analysis of Hela cell. 1, NFκB-p65 (Acetyl Lys310) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). β-Tubulin Monoclonal Antibody (5G3) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min).

Immunohistochemical analysis of paraffin-embedded Human-stomach tissue. 1, NFκB-p65 (Acetyl Lys310) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Human-stomach-cancer tissue. 1, NFκB-p65 (Acetyl Lys310) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. 1, NFκB-p65 (Acetyl Lys310) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Quick Database Links

UniProt

Q04206