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| Catalog Number | orb763969 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to NF kappa B-p65 (Acetyl Lys310) |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse |
| Concentration | 1 mg/ml |
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human NF-kappaB p65 around the acetylated site of Lys310. AA range:275-324 |
| MW | 65 |
| Tested applications | ELISA, IF, IHC-P, WB |
| Dilution range | WB 1:500-2000, IHC-p 1:50-300, IF 1:50-300 |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Anti-RELA antibody, anti-NFKB3 antibody, anti-Tran Read more... |
| Note | For research use only |
Immunofluorescence analysis of Hela cell. 1, NFκB-p65 (Acetyl Lys310) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). β-Tubulin Monoclonal Antibody (5G3) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min).
Immunohistochemical analysis of paraffin-embedded Human-stomach tissue. 1, NFκB-p65 (Acetyl Lys310) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-stomach-cancer tissue. 1, NFκB-p65 (Acetyl Lys310) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. 1, NFκB-p65 (Acetyl Lys310) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
