NFκB-p105/p50 (phospho Ser337) rabbit pAb Antibody

SKU: orb764341

Description

Rabbit polyclonal antibody to NF kappa B-p105/p50 (phospho-Ser337)

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
• Reactivity: Human, Mouse

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Synthesized phospho-peptide around the phosphorylation site of human NFκB-p105/p50 (phospho Ser337)
• Molecular Weight: 105+50
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-NFKB1 antibody, anti-Nuclear factor NF-kappa-B p105 subunit antibody, anti-DNA-binding factor KBF1 antibody, anti-EBP-1 antibody, anti-Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 antibody

Western Blot analysis of various cells using Phospho-NFκB-p105/p50 (S337) Polyclonal Antibody diluted at 1:500.

Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Immunohistochemistry analysis of paraffin-embedded human breast cancer, using NF-κB p105/p50 (Phospho-Ser337) Antibody. The picture on the right is blocked with the NF-κB p105/p50 (Phospho-Ser337) peptide.