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| Catalog Number | orb769243 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to NFATc3 (phospho-Ser165) |
| Clonality | Polyclonal |
| Tested applications | ELISA, IF, IHC-P, WB |
| Reactivity | Human, Mouse |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human NFAT4 around the phosphorylation site of Ser165. AA range:131-180 |
| Concentration | 1 mg/ml |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Conjugation | Unconjugated |
| MW | 115 |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Alternative names | Anti-NFATC3 antibody, anti-NFAT4 antibody, anti-Nu Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Immunofluorescence analysis of HeLa cells, using NFAT4 (Phospho-Ser165) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from HeLa cells treated with Ca + 40nM 30', using NFAT4 (Phospho-Ser165) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
