NFAT5 (phospho Ser155) rabbit pAb

SKU: orb767414

Description

Rabbit polyclonal antibody to NFAT5 (phospho-Ser155)

Images & Validation

• Tested Applications: ELISA, WB
• Dilution range: WB 1:500-2000;IHC-p 1:50-300
• Reactivity: Human, Mouse

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human NFAT5/TonEBP around the phosphorylation site of Ser155. AA range:121-170
• Molecular Weight: 200
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-NFAT5 antibody, anti-KIAA0827 antibody, anti-TONEBP antibody, anti-Nuclear factor of activated T-cells 5 antibody, anti-NF-AT5 antibody, anti-T-cell transcription factor NFAT5 antibody, anti-Tonicity-responsive enhancer-binding protein antibody, anti-TonE-binding protein antibody, anti-TonEBP antibody

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using NFAT5/TonEBP (Phospho-Ser155) Antibody.

Western blot analysis of lysates from HeLa cells treated with forskolin 40nM 30', using NFAT5/TonEBP (Phospho-Ser155) Antibody. The lane on the right is blocked with the phospho peptide.

Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).