NF-κB p65 Rabbit mAb [KD Validated]

• Catalog Number: orb1951958
• Category: Antibodies
• Description: NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm.
• Species/Host: Rabbit
• Clonality: Recombinant
• Tested applications: FC, ICC, IHC, IP, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Immunogen: A synthesized peptide derived from human NF-κB p65
• Antibody Type: Recombinant Antibody
• Dilution range: WB 1:5000~1:10000 IHC 1:50~1:100 ICC/IF 1:50~1:100
• Form/Appearance: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Conjugation: Unconjugated
• UniProt ID: Q04206
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: NFKB3; Transcription factor p65; p65; Nuclear fact
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.

Immunofluorescent analysis of HT-1080 cells.

Immunohistochemical analysis of paraffin-embedded human transitional cell carcinoma of bladder.

Western blot analysis expression in HeLa cell lysate.