NF-κB (p105/p50) Rabbit mAb [KD Validated] Antibody

SKU: orb1951954

Description

NFkB-p105 a transcription factor of the nuclear factor-kappaB ( NFkB) group. Undergoes cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of NFkB. NFkB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products.

Images & Validation

• Tested Applications: IHC, WB
• Dilution Range: WB 1:500~1:2000 IHC 1:50~1:200
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Recombinant Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone No.: AB9
• Immunogen: A synthesized peptide derived from human NF-κB (p105/p50)
• Target: NFKB1
• Purification: Affinity-chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NF-kappa-B1; p84/NF-kappa-B1 p98; NFKB1; DNA-binding factor KBF1; EBP-1; NFkB-p50;

All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

All Lanes: at 1/1000 dilution. Lane 1: PC-12 cell lysate. Lane 2: NIH/3T3 cell lysate. (Proteins at 20 ug per lane). Secondary: Goat Anti-Rabbit IgG (HRP) at 1/20000 dilution. Predicted band size: 50 kDa/120 kDa, Observed band size: 50 kDa/120 kDa.

Immunohistochemical analysis of paraffin-embedded human kidney. Tris-EDTA Buffer (pH9.0).

Quick Database Links

Gene Symbol

NFKB1

UniProt

P19838