NF-κB-IN-1

SKU: orb1219014

Description

NF-κB-IN-1 is a potent NF-κB signaling pathway inhibitor. It is a 4-arylidene crucumin analogue. NF-κB-IN-1 directly inhibits IKK to block NF-κB activation. NF-κB-IN-1 effectively inhibits the viability of lung cancer cells and attenuates the clonogenic activity of A549 cells.

Key Properties

• CAS Number: 1227098-15-8
• MW: 530.57
• Purity: >98% (HPLC)
• Formula: C₃₁H₃₀O₈
• SMILES: O=C(/C=C/C(C=C1)=CC(OC)=C1OC)/C(C(/C=C/C(C=C2)=CC(OC)=C2OC)=O)=C/C(C=C3)=CC(OC)=C3O
• Target: NF-κB
• Solubility: DMSO:50 mg/mL (94.24 mM; Need ultrasonic)

Bioactivity

• In Vitro:
  NF-κB-IN-1 (compound 17) (0.001-100 μM; 72 h) inhibits the growth of A549, H1944, H460 and H157 cells, with GI50s of 0.72, 0.07, 0.13, and 0.16 μM, respectively. NF-κB-IN-1 (0.5-25 μM; pretreated for 30 min or 4 h) potently blocks the IκB phosphorylation and degradation in A549 cells. NF-κB-IN-1 (0.1-100 μM; pretreated for 30 min) dose-dependently inhibits the TNFα-induced nuclear translocation of NF-κB in A549 cells, with an IC50s of 1.0 μM. NF-κB-IN-1 (0.1-0.4 μM; 9 d) inhibits lung cancer clonogenic activity. Cell Viability Assay Cell line: A549, H1944, H460 and H157 cells. Concentration: 0.001, 0.01, 0.1, 1, 10, 100 μM Incubation time: 72 hours. Result: Decreased viability of lung adenocarcinoma cells A549 and H1944, quamous cell carcinoma cells H157, and large cell carcinoma cells H460 in a dose-dependent manner. Western blot analysis. Cell line: A549 cells. Concentration: 0.5, 1.0, 2.5, 5.0, 10.0, 25.0 μM Incubation time: Pretreated for 30 min or 4 h. Result: Blocked IκB phosphorylation, with an IC50 of 2.8 μM.
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Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

1,6-Heptadiene-3,5-dione, 1,7-bis(3,4-dimethoxyphenyl)-4-[(4-hydroxy-3-methoxyphenyl)methylene]-, (1E,6E)-

Quick Database Links

Gene Symbol

NF-κB