Neuropeptide Y Receptor Y5 Antibody

• Catalog Number: orb1247207
• Category: Antibodies
• Description: Neuropeptide Y Receptor Y5 Antibody
• Target: NPY5R
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: C-GLSNKENRLEEN
• UniProt ID: Q15761
• Tested applications: ELISA, IHC, IP, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 50kDa band observed in Human Brain, Breast and Duodenum lysates (calculated MW of 50.7kDa according to NP_006165.1). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:In paraffin embedded Human Intestine shows staining of ganglion cells. Recommended concentration, 4-6ug/ml.Immunoprecipitation: Customer feedback showed specific results when this product was used in IP.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: neuropeptide Y receptor Y5 , HGNC:7958, NPY5R, NPY
• Research Area: Cancer Biology
• Note: For research use only
• NCBI: NP_006165.1

orb1247207 (0.3 ug/ml) staining of Human Brain lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247207 (3.8 ug/ml) staining of paraffin embedded Human Intestine. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.