Neuroligin 3 Antibody (HRP)

SKU: orb151031

Description

Mouse monoclonal to Neuroligin 3 (HRP). Neuroligins are Type I membrane proteins enriched in synaptic plasma membranes and clustered in synaptic clefts and postsynaptic densities. They have been characterized as neuronal cell surface proteins and are thought to be involved in cell-cell-interactions by forming intercellular junctions through binding to beta-neurexins. They play a major role in the formation or maintenance of synaptic junctions. They are also thought to be involved in the specification of excitatory synapses. Neuroligins interact with beta-neurexins and this interaction is involved in the formation of functional synapses..

Research Area

Disease Biomarkers, Neuroscience

Images & Validation

• Tested Applications: ICC, IF, IHC, WB
• Dilution Range: WB (1:1000)
• Reactivity: Human, Mouse, Rat
• Application Notes:
  1 µg/ml of SMC-471 was sufficient for detection of Neuroligin 3 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: N110/29 (Formerly sold as S110-29)
• Immunogen: Fusion protein amino acids 730-848 (intracellular C-terminus) of rat Neuroligin-3. Mouse: 99% identity (118/119 amino acids identical). Human: 98% identity (116/119 amino acids identical) ~60% identity with Neuroligin-1. ~40% identity with Neuroligin-2.
• Target: Neuroligin 3
• Molecular Weight: 110kDa
• Purification: Protein G Purified
• Conjugation: HRP

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide 0.23mM Citrate
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Gliotactin homolog, Neuroligin-3, Nlgn3, NLGN3_HUMAN

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Neuroligin 3 Monoclonal Antibody, Clone N110/29. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Neuroligin 3 Monoclonal Antibody at 1:50 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Neuroligin 3 Antibody (D) Composite.

Quick Database Links

Gene Symbol

Neuroligin 3

UniProt

Q62889

NCBI Gene ID

171297

RefSeq:AAA97871