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Neurofilament NF-H, phosphylated Antibody
SKU: orb176259
Description
Research Area
Neuroscience
Images & Validation
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Item 1 of 2
| Tested Applications | ICC, IHC, WB |
|---|---|
| Dilution Range | ICC 1:1000; IHC 1:1000; WB 1:10,000 |
| Reactivity | Gallus, Human, Mouse, Primate, Rat |
Key Properties
−| Host | Rabbit |
|---|---|
| Isotype | Rabbit IgG |
| Immunogen | Bovine intermediate filaments were prepared from spinal cords by the method of Delacourte et al. and the cytoskeletal material was dissolved in 6M urea. Individual neurofilament subunits were purified by ion exchange chromatography on DEAE cellulose followed by preparative gel electrophoresis. The form of NF-H isolated is the heavily phosphorylated axonal variant, and the antibody generated recognizes predominantly axonal neurofilaments. |
| Purification | Whole serum |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Whole serum |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Quality Guarantee
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ICC
Immunocytochemistry
Available Sizes
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