Neurofilament light polypeptide (NF-L) Antibody (DA2)

SKU: orb3167409

Description

Our Anti-Neurofilament light polypeptide (NF-L) mouse monoclonal primary antibody detects bovine, horse, human, mouse, pig, and rat Neurofilament light polypeptide (NF-L), and is IgG. It is validated for use in FC, IF, ICC, IHC, WB.

Images & Validation

• Tested Applications: FC, ICC, IF, IHC, WB
• Dilution Range: WB: 1:5000; IHC: 1:2000; ICC: 1:1000
• Reactivity: Bovine, Equine, Human, Mouse, Porcine, Rat
• Application Notes:
  A dilution of 2 ug per 10^6 cells is recommended for FC.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: DA2
• Immunogen: The antibody has been made against enzymatically dephosphorylated, full length, pig NF-L protein. The antibody binding epitope has been mapped to a short peptide in the C-terminal “tail” region of the molecule within the sequence YYTSHVQEEQIEVEETIEA, amino acids 441-460 of the human sequence.
• Target: Neurofilament light polypeptide (NF-L)
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NF-L; NF68; NEFL; Neurofilament light polypeptide; Neurofilament protein; Neurofilament triplet L protein; NFL

Quick Database Links

Gene Symbol

Neurofilament light polypeptide (NF-L)

UniProt

P07196