NCDN Antibody

SKU: orb1247214

Description

NCDN Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 75kDa band observed in lysates of cell lines HepG2 and U251 (calculated MW of 77.2kDa according to NP_001014841.1). An additional band of unknown identity was also observed in U251 lysates at 55kDa. This band was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 0.3-1µg/mL. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Rat and Mouse Kidney and Brain and NIH3T3 cell line for this particular batch.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-HYLLQVGSEKQKEP
• Target: NCDN
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NCDN, neurochondrin , KIAA0607

orb1247214 (0.3 ug/ml) staining of HepG2 (A) and (1 ug/ml) U251 cell lysate (B) + peptide (C) (35 ug protein in RIPA buffer). Detected by chemiluminescence.

Quick Database Links

Gene Symbol

NCDN

UniProt

Q9UBB6

RefSeq (Protein):NP_055099.1, NP_001014841.1