Neurochondrin Antibody

SKU: orb19396

Description

Goat polyclonal antibody to NCDN

Images & Validation

• Tested Applications: ELISA, WB
• Dilution range: ELISA: 1:16000, WB: 0.3-1 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Preliminary experiments gave bands at approx 100kDa and140kDa in Human Brain (Cerebellum) lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 78.9kDa according to NP_055099.1 and 77.2kDa according to NP_001014841.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: Neurochondrin
• Molecular Weight: 78.9; 77.2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti NCDN antibody, anti neurochondrin antibody, anti KIAA0607 antibody

WB analysis of HepG2 (A), U251 cell lysate (B) using NCDN antibody

Quick Database Links

Gene Symbol

Neurochondrin

NCBI Gene ID

23154