NBEA Antibody

SKU: orb1247215

Description

NBEA Antibody

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 300kDa band observed in lysates of transfected HEK293 and endogenously in lysates of insulinoma cell line B-TC3 (calculated MW of 327kDa according to NP_056493.3). Data from a previous goat. Data kindly provided by Professor John Creemers, K.U. Leuven, Belgium. Recommended concentration: 1-3ug/ml.Immunohistochemistry:In paraffin embedded Human Cerebral Cortex shows textured cytoplasm staining in the neuronal cell bodies. Recommended concentration: 5-10ug/ml.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-DFNRWHYEHQNRY
• Target: NBEA
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Disclaimer: For research use only

Alternative Names

NBEA, neurobeachin, RP11-270C18.1, BCL8B, FLJ10197, KIAA1544, LYST2, lysosomal trafficking regulator, lysosomal trafficking regulator 2

orb1247215 (1 ug/ml) staining of 1) untransfected HEK293T cells 2) HEK293T cells transfected with mouse NBEA. Detected by chemiluminescence.

orb1247215 (5 ug/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

NBEA

UniProt

Q8NFP9

RefSeq (Protein):NP_001191126.1, NP_056493.3