Neurexin 1 Antibody

• Catalog Number: orb1246691
• Category: Antibodies
• Description: Neurexin 1 Antibody
• Target: NRXN1
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: C-KEKQPSSAKSSNKN
• UniProt ID: P58400
• Tested applications: ELISA, FC, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:In transfected HEK293 transiently expressing full-length Human NRXN1 (myc and DYKDDDDK tagged), bands of approx. 75 and 60kDa were observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody. Recommended concentration: 0.5-1ug/ml. Primary incubations were for 1 hour.Flow Cytometry: Flow cytometric analysis of Kelly cells. Recommended concentration: 10ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: neurexin 1, DKFZp313P2036, NRXN1, FLJ35941, Hs.229
• Research Area: Neuroscience
• Note: For research use only
• NCBI: NP_004792.1, NP_620072.1

HEK293 lysate (10 ug protein in RIPA buffer) overexpressing Human NRXN1 with DYKDDDDK tag probed with orb1246691 (0.5 ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/3000) in lane C. Mock-transfected HEK293 probed with orb1246691 (1 mg/ml) in Lane B.

orb1246691 Flow cytometric analysis of paraformaldehyde fixed Kelly cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line).